Liquid chromatography
puriFlash 1 — Interchim puriFlash 4250
Interchim puriFlash 4250 for chromatographic purification and fraction collection. The puriFlash 1 station uses InterSoft 5.0f.07.
Demo and booking provide different access
The public demo covers recorded chromatogram analysis. It does not start pumps, inject samples or acquire measurements. The laboratory defines real instrument access in the accepted offer.
Equipment includes a MACHEREY-NAGEL VP 250/40 NUCLEODUR 100-5 C18 ec preparative column (250 × 40 mm, 5 µm) for reversed-phase separations. When booking, specify sample type and quantity, purification goals and any existing method. Indicate whether you need an operator and use of the C18 column. The laboratory confirms the configuration, dates, column use conditions, solvents and fraction delivery. Operator support, materials and a complete purification service require an individual quote. The free demo runs offline on sample data and does not control the instrument.
From separation to collected fractions
An introduction to InterSoft functions. The laboratory agrees the instrument configuration and materials for each purification.
- Gradient and flow
- Review the elution programme and solvent proportions.
- UV detection
- Explore recorded signals and compare the separation trace.
- Fraction collection
- Link chromatogram regions to collection tubes and the collection table.
- Methods and data
- Explore a sample separation in InterSoft 5.0f.07 in OFF LINE mode.
Instrument and column
Physical equipment of the puriFlash 1 station. Demo sample data do not establish that the separation used this column.
- Model
- Interchim puriFlash 4250 · PF4250-A-10-C
- Preparative column
- MACHEREY-NAGEL VP 250/40 NUCLEODUR 100-5 C18 ec
- Dimensions and particle size
- 250 × 40 mm · 5 µm
- Phase
- C18 ec · RP · endcapped
- Catalogue number
- 762022.400
- SN / LOT · NUCLEODUR
- E11020679 / 3450043
- Equipment
- Touchscreen, fraction collector, column holder and injection valve.
Rental prices
| Duration | Price per day |
|---|---|
| 1 day | €600 |
| 2–30 days | €450 |
| From 31 days | €299 |
The rate for the selected duration applies to every rental day.